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pcna  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pcna
    Pcna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/13110s/PCNA+XP+Rabbit+mAb/pmc12974335-132-43-44
    Average 97 stars, based on 1149 article reviews
    pcna - by Bioz Stars, 2026-09
    97/100 stars

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    other:


    Article Title: Artificial targeting of the NEIL1 DNA glycosylase to the mitochondria
    Article Snippet: PCNA antibody , Cell Signaling Technologies , 13110S.

    Article Title: Induced hepatocyte-like cells derived from adipose-derived stem cells alleviates liver injury in mice infected with Echinococcus Multilocularis
    Article Snippet: Experiment Antibody Supplier Catalog number Dilution concentration FITC Mouse anti Human CD31 BD, USA 555445 1:10 PE Mouse anti Human CD34 BD, USA 550781 1:10 FITC Mouse anti Human CD45 BD, USA 555482 1:10 Flow cytometry PE Mouse anti Human CD29 eBioscience 12-0299-42 1:40 FITC Mouse anti Human CD44 BD, USA 555478 1:10 PE Mouse anti Human CD90 eBioscience 12-0909-42 1:40 PEMouse anti Human HLA-DR BD, USA 568230 1:10 Chk pAb to ALB abcam,USA ab106582 1∶500 Rabbit pAb to CD29 Affinity,China AF5379 1∶200 Rabbit pAb to CD44 Affinity,China DF6392 1∶200 Rabbit pAb to CD90 Affinity,China DF6849 1∶200 Immunofluorescence Rabbit pAb to CD105 Affinity,China DF7431 1∶200 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) abcam,USA ab150169 1∶250 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) abcam,USA ab150077 1∶250 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) abcam,USA ab150080 1∶250 Rabbit mAb to β-catenin CST, USA 8480S 1∶1000 Rabbit pAb to Collagen I abcam,USA ab34710 1∶1000 Western Blot Rabbit mAb to GAPDH abcam,USA ab181602 1∶5000 Goat Anti-Rabbit IgG(H+L)/HRP ZSGB-BIO, China ZB2301 1∶5000 Rabbit pAb to CD31 abcam,USA ab28364 1∶50 Rabbit mAb to PCNA CST, USA 13110S 1∶1000 Immunohistochemistry Rabbit mAb to α-SMA abcam,USA ab124964 1∶1000 Goat Anti-Rabbit IgG H&L (HRP) abcam,USA ab97051 1∶500 S2 Table.

    Article Title: Inhibition of XPO1 with KPT-330 induces autophagy-dependent apoptosis in gallbladder cancer by activating the p53/mTOR pathway
    Article Snippet: PCNA , 13110S , 1:2000 , Cell Signaling Technology, USA.

    Incubation:

    Article Title: Accumulation of polyunsaturated lipids fuels ferroptosis to promote liver failure after extended hepatectomy in mice.
    Article Snippet: Background: Post-hepatectomy liver failure (PhlF) is a fatal complication of hepatectomy. however, the mechanism of hepatocyte injury in PhlF remains elusive.. Methods: PhlF was induced by extended 86% hepatectomy (ehx) in mice. lipidomics was performed to investigate the ehx-induced lipid alteration in the residual liver.. Ferroptosis was assessed to screen the hepatocyte injury induced by ehx. the therapeutic effects of ferrostatin-1 (Fer-1) on PhlF were evaluated.

    Article Title: MAGED4B Promotes Glioma Progression via Inactivation of the TNF-α-induced Apoptotic Pathway by Down-regulating TRIM27 Expression.
    Article Snippet: cisplatin and temozolomide.. MAGED4B knockdown also inhibited the growth of gliomas implanted into the rat brain.. The interaction between MAGED4B and tripartite motifcontaining 27 (TRIM27) in glioma cells was detected by coimmunoprecipitation assay, which showed that MAGED4B was co-localized with TRIM27.

    Western Blot:

    Article Title: CDK-independent role of D-type cyclins in regulating DNA mismatch repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FLAG (1:2000) Sigma-Aldrich Cat#F1804; RRID: AB_262044 FLAG (1:2000) Sigma-Aldrich Cat#F7425; RRID: AB_439687 GAPDH (1:5000) Cell Signaling Technology Cat#97166S; RRID: AB_2756824 GFP (1:1000) Cell Signaling Technology Cat#2956S; RRID: AB_1196615 GST (1:2000) Proteintech Cat#66001-2-Ig; RRID: AB_2881488 HA (1:2000) Bethyl Laboratories Cat#A190-108A; RRID: AB_67465 HA (1:5000) Covance Cat#MMS-101P; RRID: AB_2314672 Histone H3 (1:10000) Abcam Cat#ab1791; RRID: AB_302613 KAP1 (1:2000) Proteintech Cat#66630-1-Ig; RRID: AB_2732886 LIG1 (1:1000) Proteintech Cat#18051-1-AP; RRID: AB_2265726 LIG3 (1:1000) Proteintech Cat#26583-1-AP; RRID: AB_2880562 MLH1(1:1000) Cell Signaling Technology Cat#3515S; RRID: AB_2145615 MLH1 (1:1000) Cell Signaling Technology Cat#4256S; RRID: AB_10545447 Mouse Gamma Globulin Control ThermoFisher Scientific Cat#31878; RRID: AB_2532170 MSH2 (1:1000) Cell Signaling Technology Cat#2017S; RRID: AB_2235387 MSH2 (1:1000) Cell Signaling Technology Cat#2850S; RRID: AB_2144797 MSH3 (1:1000) Proteintech Cat#22393-1-AP; RRID: AB_11232405 MSH6 (1:1000) Cell Signaling Technology Cat#12988S; RRID: AB_2750881 MSH6 (1:1000) Cell Signaling Technology Cat#5424S; RRID: AB_10695802 Myc (1:2000) Cell Signaling Technology Cat#2278S; RRID: AB_490778 OGG1 (1:1000) Novus Biologicals Cat#NB100-106; RRID: AB_10104097 p21 (1:1000) Cell Signaling Technology Cat#2947S; RRID: AB_823586 p21 (1:1000) Proteintech Cat#28248-1-AP; RRID: AB_2881097 p27 (1:1000) BD Biosciences Cat#610241; RRID: AB_397636 p27 (1:1000) Cell Signaling Technology Cat#3686S; RRID: AB_2077850 p57 (1:1000) Cell Signaling Technology Cat#2557S; RRID: AB_2291591 p57 (1:1000) Proteintech Cat#66794-1-Ig; RRID: AB_2882138 (Continued on next page) e2 Molecular Cell 84, 1224–1242.e1–e13, April 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER P57 (1:1000) Proteintech Cat#23317-1-AP; RRID: AB_2879254 PARP1 (1:1000) Cell Signaling Technology Cat#9542S; RRID: AB_2160739 pATM (S1981) (1:1000) Cell Signaling Technology Cat#4526L; RRID: AB_2062663 pCHK2 (T68) (1:1000) Cell Signaling Technology Cat#2661S; RRID: AB_331479 pKAP1 (S824) (1:1000) Bethyl Laboratories Cat#A300-767A; AB_669740 PCNA (1:2000) Cell Signaling Technology Cat13110S; RRID: AB_2636979 PCNA (1:2000 or 10 mg for IP) Thermo Fisher Scientific Cat#14-9910-37; RRID: AB_2865515 pH3 (S10) (1:2000) Cell Signaling Technology Cat#3377S; RRID: AB_1549592 POLB (1:2000) Proteintech Cat#18003-1-AP; RRID: AB_2299845 pRB (S780) (1:1000) Cell Signaling Technology Cat#9307S; RRID: AB_330015 pRB (S807/811) (1:1000) Cell Signaling Technology Cat#8516S; RRID: AB_11178658 RB (1:1000) Cell Signaling Technology Cat#9309S; RRID: AB_823629 RB (1:1000) Cell Signaling Technology Cat#9313S; RRID: AB_1904119 RPA2 (1:1000 for IF) Cell Signaling Technology Cat# 2208S; RRID:AB_2238543 RPA2 (1:1000 for WB) EMD Millipore Cat#NA19L; RRID:AB_565123 SKP2 (1:1000) Cell Signaling Technology Cat#2652S; RRID: AB_11178941 TP53BP1 (1:2000) Abcam Cat#ab36823; RRID: AB_722497 Vinculin (1:2000) Bethyl Laboratories Cat#A302-535A; RRID: AB_1999080 XPA (1:1000) Abcam Cat#ab65963; RRID: AB_1144269 XRCC1 (1:1000) Cell Signaling Technology Cat#76998S; RRID: AB_2936252 XRCC1 (1:1000) Proteintech Cat#21468-1-AP; RRID: AB_2878865 aTubulin (1:5000) Sigma-Aldrich Cat#T6074; RRID: AB_477582 gH2A.X (1:1000) Cell Signaling Technology Cat#9718S; RRID: AB_2118009 gH2A.X (1:1000) EMD Millipore Cat#05-636; RRID: AB_309864 Bacterial and virus strains NEB5-alpha New England Biolabs Cat#C2992H NEB Stable New England Biolabs Cat#C3040H (Continued on next page) Molecular Cell 84, 1224–1242.e1–e13, April 4, 2024 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Neocarzinostatin (0.2 mg/ml) (NCS) Sigma-Aldrich Cat#N9162-100UG Bleomycin (20 mg/ml) Selleck Cat#S1214 H2O2 (40-250 mM) Sigma-Aldrich Cat#H1009-100ML MMS (500 mM) Sigma-Aldrich Cat#129925-5G Methylnitronitrosoguanidine (10 mM) (MNNG) TCI Cat#M0527 O6-benzylguanine (10 mM) Sigma-Aldrich Cat#Cat#B2292 Camptothecin (1 mM) (CPT) EMD Millipore Cat#208925-50MG Cycloheximide (100 mg/ml) Sigma-Aldrich Cat#C7698-1G Doxycycline (1 mg/ml) Sigma-Aldrich Cat#D9891-1G EdU (5-Ethynyl-2’-deoxyuridine) (10 mM) Thermo Fisher Scientific Cat#1149-100 BrdU (5-Bromo-2’-deoxyuridine) (10 mM) Merck Cat#19-160 Hoechst 33342 (1 mg/mL) Thermo Fisher Scientific Cat#H3570 MG132 (10 mM) Peptides International Cat#IZL-3175v Lipofectamine 3000 Invitrogen Cat#L3000150 Lipofectamine RNAi Max Invitrogen Cat#13778-500 MLN4924 (2.5 mM) Active Biochem Cat#A-1139 Polybrene (8 mg/mL) Sigma-Aldrich Cat#TR-1003 Puromycin (2 mg/mL) Sigma-Aldrich Cat#P9620 Hygromycin (300 mg/mL) Roche Cat#10843555001 Blasticidin (5 mg/ml) Invivogen Cat#ant-bl-05 Palbociclib (1 mM) Selleck Chemicals Cat#S1579 GST (0.2 mg/ml) SinoBio Cat#11213-HNAE GST-p21 (0.2 mg/ml) Abcam Cat#ab56278 His-CCND1 (0.2 mg/ml) LSBio Cat# LS-G20572-100 His-CDK4 (0.2 mg/ml) LSBio Cat#LS-G146214-100 His-CCNA2 (0.5 mg/ml) Abcam Cat#ab126696 CDK2-His (0.5 mg/ml) Abcam Cat#ab111646 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Thermo Fisher Scientific Cat#C10640 Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat#C10632 FxCycle PI/RNase Staining Solution Thermo Fisher Scientific Cat#F10797 Click-iT Plus Picolyl Azide Toolkit Thermo Fisher Scientific Cat#C10641 CometAssay Single Cell Gel Electrophoresis Assay R&D Systems (Trevigen) Cat#4250-050-K NanoGlo Luciferase Assay System Promega Cat#N1130 Alamarblue Reagent BioRad Cat#BUF012B P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Deposited data MassIVE: MSV000091006 (proteomics) This study https://massive.ucsd.edu/ ProteomeXchange Consortium: PXD039228 (proteomics) This study http://www.proteomexchange.org/ Mendeley Data: https://doi.org/10.17632/ fkbfsms225.1 (original western blot images) This study https://data.mendeley.com/ datasets/fkbfsms225/1 (Continued on next page) e4 Molecular Cell 84, 1224–1242.e1–e13, April 4, 2024

    Virus:

    Article Title: CDK-independent role of D-type cyclins in regulating DNA mismatch repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER FLAG (1:2000) Sigma-Aldrich Cat#F1804; RRID: AB_262044 FLAG (1:2000) Sigma-Aldrich Cat#F7425; RRID: AB_439687 GAPDH (1:5000) Cell Signaling Technology Cat#97166S; RRID: AB_2756824 GFP (1:1000) Cell Signaling Technology Cat#2956S; RRID: AB_1196615 GST (1:2000) Proteintech Cat#66001-2-Ig; RRID: AB_2881488 HA (1:2000) Bethyl Laboratories Cat#A190-108A; RRID: AB_67465 HA (1:5000) Covance Cat#MMS-101P; RRID: AB_2314672 Histone H3 (1:10000) Abcam Cat#ab1791; RRID: AB_302613 KAP1 (1:2000) Proteintech Cat#66630-1-Ig; RRID: AB_2732886 LIG1 (1:1000) Proteintech Cat#18051-1-AP; RRID: AB_2265726 LIG3 (1:1000) Proteintech Cat#26583-1-AP; RRID: AB_2880562 MLH1(1:1000) Cell Signaling Technology Cat#3515S; RRID: AB_2145615 MLH1 (1:1000) Cell Signaling Technology Cat#4256S; RRID: AB_10545447 Mouse Gamma Globulin Control ThermoFisher Scientific Cat#31878; RRID: AB_2532170 MSH2 (1:1000) Cell Signaling Technology Cat#2017S; RRID: AB_2235387 MSH2 (1:1000) Cell Signaling Technology Cat#2850S; RRID: AB_2144797 MSH3 (1:1000) Proteintech Cat#22393-1-AP; RRID: AB_11232405 MSH6 (1:1000) Cell Signaling Technology Cat#12988S; RRID: AB_2750881 MSH6 (1:1000) Cell Signaling Technology Cat#5424S; RRID: AB_10695802 Myc (1:2000) Cell Signaling Technology Cat#2278S; RRID: AB_490778 OGG1 (1:1000) Novus Biologicals Cat#NB100-106; RRID: AB_10104097 p21 (1:1000) Cell Signaling Technology Cat#2947S; RRID: AB_823586 p21 (1:1000) Proteintech Cat#28248-1-AP; RRID: AB_2881097 p27 (1:1000) BD Biosciences Cat#610241; RRID: AB_397636 p27 (1:1000) Cell Signaling Technology Cat#3686S; RRID: AB_2077850 p57 (1:1000) Cell Signaling Technology Cat#2557S; RRID: AB_2291591 p57 (1:1000) Proteintech Cat#66794-1-Ig; RRID: AB_2882138 (Continued on next page) e2 Molecular Cell 84, 1224–1242.e1–e13, April 4, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER P57 (1:1000) Proteintech Cat#23317-1-AP; RRID: AB_2879254 PARP1 (1:1000) Cell Signaling Technology Cat#9542S; RRID: AB_2160739 pATM (S1981) (1:1000) Cell Signaling Technology Cat#4526L; RRID: AB_2062663 pCHK2 (T68) (1:1000) Cell Signaling Technology Cat#2661S; RRID: AB_331479 pKAP1 (S824) (1:1000) Bethyl Laboratories Cat#A300-767A; AB_669740 PCNA (1:2000) Cell Signaling Technology Cat13110S; RRID: AB_2636979 PCNA (1:2000 or 10 mg for IP) Thermo Fisher Scientific Cat#14-9910-37; RRID: AB_2865515 pH3 (S10) (1:2000) Cell Signaling Technology Cat#3377S; RRID: AB_1549592 POLB (1:2000) Proteintech Cat#18003-1-AP; RRID: AB_2299845 pRB (S780) (1:1000) Cell Signaling Technology Cat#9307S; RRID: AB_330015 pRB (S807/811) (1:1000) Cell Signaling Technology Cat#8516S; RRID: AB_11178658 RB (1:1000) Cell Signaling Technology Cat#9309S; RRID: AB_823629 RB (1:1000) Cell Signaling Technology Cat#9313S; RRID: AB_1904119 RPA2 (1:1000 for IF) Cell Signaling Technology Cat# 2208S; RRID:AB_2238543 RPA2 (1:1000 for WB) EMD Millipore Cat#NA19L; RRID:AB_565123 SKP2 (1:1000) Cell Signaling Technology Cat#2652S; RRID: AB_11178941 TP53BP1 (1:2000) Abcam Cat#ab36823; RRID: AB_722497 Vinculin (1:2000) Bethyl Laboratories Cat#A302-535A; RRID: AB_1999080 XPA (1:1000) Abcam Cat#ab65963; RRID: AB_1144269 XRCC1 (1:1000) Cell Signaling Technology Cat#76998S; RRID: AB_2936252 XRCC1 (1:1000) Proteintech Cat#21468-1-AP; RRID: AB_2878865 aTubulin (1:5000) Sigma-Aldrich Cat#T6074; RRID: AB_477582 gH2A.X (1:1000) Cell Signaling Technology Cat#9718S; RRID: AB_2118009 gH2A.X (1:1000) EMD Millipore Cat#05-636; RRID: AB_309864 Bacterial and virus strains NEB5-alpha New England Biolabs Cat#C2992H NEB Stable New England Biolabs Cat#C3040H (Continued on next page) Molecular Cell 84, 1224–1242.e1–e13, April 4, 2024 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Neocarzinostatin (0.2 mg/ml) (NCS) Sigma-Aldrich Cat#N9162-100UG Bleomycin (20 mg/ml) Selleck Cat#S1214 H2O2 (40-250 mM) Sigma-Aldrich Cat#H1009-100ML MMS (500 mM) Sigma-Aldrich Cat#129925-5G Methylnitronitrosoguanidine (10 mM) (MNNG) TCI Cat#M0527 O6-benzylguanine (10 mM) Sigma-Aldrich Cat#Cat#B2292 Camptothecin (1 mM) (CPT) EMD Millipore Cat#208925-50MG Cycloheximide (100 mg/ml) Sigma-Aldrich Cat#C7698-1G Doxycycline (1 mg/ml) Sigma-Aldrich Cat#D9891-1G EdU (5-Ethynyl-2’-deoxyuridine) (10 mM) Thermo Fisher Scientific Cat#1149-100 BrdU (5-Bromo-2’-deoxyuridine) (10 mM) Merck Cat#19-160 Hoechst 33342 (1 mg/mL) Thermo Fisher Scientific Cat#H3570 MG132 (10 mM) Peptides International Cat#IZL-3175v Lipofectamine 3000 Invitrogen Cat#L3000150 Lipofectamine RNAi Max Invitrogen Cat#13778-500 MLN4924 (2.5 mM) Active Biochem Cat#A-1139 Polybrene (8 mg/mL) Sigma-Aldrich Cat#TR-1003 Puromycin (2 mg/mL) Sigma-Aldrich Cat#P9620 Hygromycin (300 mg/mL) Roche Cat#10843555001 Blasticidin (5 mg/ml) Invivogen Cat#ant-bl-05 Palbociclib (1 mM) Selleck Chemicals Cat#S1579 GST (0.2 mg/ml) SinoBio Cat#11213-HNAE GST-p21 (0.2 mg/ml) Abcam Cat#ab56278 His-CCND1 (0.2 mg/ml) LSBio Cat# LS-G20572-100 His-CDK4 (0.2 mg/ml) LSBio Cat#LS-G146214-100 His-CCNA2 (0.5 mg/ml) Abcam Cat#ab126696 CDK2-His (0.5 mg/ml) Abcam Cat#ab111646 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Thermo Fisher Scientific Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Thermo Fisher Scientific Cat#C10640 Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit Thermo Fisher Scientific Cat#C10632 FxCycle PI/RNase Staining Solution Thermo Fisher Scientific Cat#F10797 Click-iT Plus Picolyl Azide Toolkit Thermo Fisher Scientific Cat#C10641 CometAssay Single Cell Gel Electrophoresis Assay R&D Systems (Trevigen) Cat#4250-050-K NanoGlo Luciferase Assay System Promega Cat#N1130 Alamarblue Reagent BioRad Cat#BUF012B P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Deposited data MassIVE: MSV000091006 (proteomics) This study https://massive.ucsd.edu/ ProteomeXchange Consortium: PXD039228 (proteomics) This study http://www.proteomexchange.org/ Mendeley Data: https://doi.org/10.17632/ fkbfsms225.1 (original western blot images) This study https://data.mendeley.com/ datasets/fkbfsms225/1 (Continued on next page) e4 Molecular Cell 84, 1224–1242.e1–e13, April 4, 2024

    Membrane:

    Article Title: MAGED4B Promotes Glioma Progression via Inactivation of the TNF-α-induced Apoptotic Pathway by Down-regulating TRIM27 Expression.
    Article Snippet: cisplatin and temozolomide.. MAGED4B knockdown also inhibited the growth of gliomas implanted into the rat brain.. The interaction between MAGED4B and tripartite motifcontaining 27 (TRIM27) in glioma cells was detected by coimmunoprecipitation assay, which showed that MAGED4B was co-localized with TRIM27.



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